Table 1 Primer sequences and annealing temperatures
Name | Sequences (5′-3′) | Annealing temperature(°C) |
|---|---|---|
ERG3-F | GATCATAACTCAATATGG | 60 |
ERG3-R | CTGAACACTGAATCG | |
ERG11-F | ATGGATATCGTACTAGAA | 60 |
ERG11-R | TCATTGTTCAACATATTC | |
18S-F | TCTTTCTTGATTTTGTGGGTGG | 58 |
18S-R | TCGATAGTCCCTCTAAGAAGTG | |
CDR1-F | GCTGGTGAAGGTTTGAATGT | 60 |
CDR1-R | CGCTGATGGTTGATGGATAG | |
CDR2-F | ATCTGGTGCTGGTAAGAC | 54 |
CDR2-R | GCTGATGGTTGATGGATAG |