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Table 3 Test accuracy for various routine diagnostic tests, Xpert Ultra and the MPT64 test using a composite reference standard

From: Diagnosis of extrapulmonary tuberculosis using the MPT64 antigen detection test in a high-income low tuberculosis prevalence setting

 Test performed on number of samplesSensitivity %
(95% CI)
Specificity %
(95% CI)
Overall accuracy
%
All samples (n = 274)
 Microscopy16622 (9–42)95 (90–98)83
 Culture25262 (41–80)100 (98–100)96
 PCR (1-step PCR and n-PCR pooled)8744 (26–65)100 (94–100)83
 Xpert Ultra4857 (18–90)98 (87–100)92
 MPT64 test27432 (17–51)84 (79–88)78
Lymph node biopsies (n = 24)
 Microscopy1917 (0–64)92 (64–100)68
 Culture2033 (4–78)100 (77–100)80
 PCR (1-step PCR and n-PCR pooled)1529 (4–71)100 (63–100)67
 Xpert Ultra2N/A100 (16–100)
 MPT64 test2443 (10–82)100 (80–100)83
All biopsies (n = 88)
 Microscopy6320 (4–48)94 (83–99)76
 Culture6850 (23–77)100 (93–100)90
 PCR (1-step PCR and n-PCR pooled)5737 (16–62)100 (91–100)79
 Xpert Ultra10N/A90 (56–100)
 MPT64 test8837 (16–62)99 (92–100)85
Lymph node aspirates (n = 11)
 Microscopy943 (10–82)50 (1–99)44
 Culture971 (29–96)100 (16–100)78
 PCR (1-step PCR and n-PCR pooled)967 (22–96)100 (29–100)78
 Xpert Ultra575 (19–99)100 (3–100)80
 MPT64 test1129 (4–71)50 (7–93)36
 Pus samples (n = 15)
 Microscopy150 (0–84)77 (46–95)67
 Culture1550 (1–99)100 (75–100)93
 PCR (1-step PCR and n-PCR pooled)6100 (3–100)100 (48–100)100
 Xpert Ultra1050 (1–99)100 (63–100)90
 MPT64 test1550 (1–99)69 (39–91)67
Fluid samples (n = 160)
 Microscopy790 (0–71)100 (95–100)96
 Culture160100 (29–100)100 (98–100)100
 PCR (1-step PCR and n-PCR pooled)150 (0–98)100 (77–100)93
 Xpert Ultra230 (0–98)100 (85–100)96
 MPT64 test1600 (0–71)80 (72–86)78
  1. Abbreviations: PCR, polymerase chain reaction; CI, confidence interval