Fig. 2
From: Fatal acute Chagas disease by Trypanosoma cruzi DTU TcI, Ecuador
T. cruzi genotyping by mini-exon multiplex PCR. Parasite genotyping was performed using the multiplex PCR assay targeting the mini-exon gene, which differentiates TcI from the other T. cruzi DTUs. L: DNA molecular weight ladder, with the corresponding molecular weights in base-pairs indicated on the left of the gel. P: DNA isolated from parasites cultured from the patient’s blood. TcI. DTU TcI control DNA (Cutia Cl1 strain), yielding a 200 bp band. TcII-V-VI. Control DNA from DTU TcII DNA (Tu18Cl93 strain), yielding a 250 bp band, corresponding to DTUs TcII, V or VI. TcIII-IV. DTU TcIV (CAN III Cl1 strain), yielding a 150 bp band, corresponding to DTUs III or IV. NC. Negative (no template) control. Tr. T. rangeli control DNA, yielding a 100 bp band