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Table 1 Primers for uropathogenic Escherichia coli virulence genes PCR assay [12, 16]

From: Distribution of virulence genes and phylogenetics of uropathogenic Escherichia coli among urinary tract infection patients in Addis Ababa, Ethiopia

Virulence factorTarget gene(s)Primer NamePrimer Sequence (5′- 3′)Size of amplicon (bp)
Type 1 fimbriaefimHfimH-f5′-AACAGCGATGATTTCCAGTTTGTGTG-3′465
fimH-r5′-ATTGCGTACCAGCATTAGCAATGTCC-3′
P fimbriaepapCpap15′-GACGGCTGTACTGCAGGGTGTGGCG-3’328
pap25′-ATATCCTTTCTGCAGGGATGCAATA-3’
S and FIC fimbriaeSfa/focDEh regionsfa15′-CTCCGGAGAACTGGGTGCATCTTAC-3’410
sfa25′-CGGAGGAGTAATTACAAACCTGGCA-3’
Afa adhesinsafaCcafa-f5′-CGGCTTTTCTGCTGAACTGGCAGGC-3’672
afa-r5′-CCGTCAGCCCCCACGGCAGACC-3’
HemolysinhlyCA regionhly s5′-AGATTCTTGGGCATGTATCCT-3’556
hly as5′-TTGCTTTGCAGACTGTAGTGT-3’
Cytotoxic necrotizing factorcnfcnf s5′-TTATATAGTCGTCAAGATGGA-3’693
cnf as5′-CACTAAGCTTTACAATATTGA-3’
AerobactiniucCaer s5′-AAACCTGGCTTACGCAACTGT-3’269
aer as5′-ACCCGTCTGCAAATCATGGAT-3’