Table 1 Reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) primers for detection of influenza subtypes
Target | Gene | Primer name | Sequence(5′-3′) | Primer final concentration | Gene position | Length |
|---|---|---|---|---|---|---|
(mer) | ||||||
B | NA | B-F3 | CAGGAAGAGTAAAACATACTGAGGA | 5 μM | 856–881 | 25 |
B-B3 | GATTCGCAAGGCCCTGTT | 5 μM | 1052–1069 | 18 | ||
B-FIP | AGGGTCTTTTTGCTGTGTAACTGTT-GCACATGCGGATTTGCCAG | 40 μM | 933–955 + 883–901 | 44 | ||
B-BIP | GTGGAGACTGATACAGCGGAA-TGCTTCCATCATTTGGTCTGG | 40 μM | 972–992 + 1030–1050 | 42 | ||
B-LF | CTACAGGCACATTCTATGGTT | 10 μM | 908–928 | 21 | ||
B-LB | ATAAGATTGATGTGCACA | 10 μM | 993–1010 | 18 | ||
A/H1 | HA | H1-F3 | AGCAAGAAGTTCAAGCCG | 5 μM | 619–639 | 18 |
H1-B3 | CGTGAACTGGTGTATCTGAA | 5 μM | 801–820 | 20 | ||
H1-FIP | GGCTCTACTAGTGTCCAGTAATAGT-AAATAGCAATAAGACCCAAAGTG | 80 μM | 734–758 + 689–711 | 48 | ||
H1-BIP | ATAACATTCGAAGCAACTGGAAATC-TGATAATACCAGATCCAGCATT | 80 μM | 718–742 + 778–799 | 47 | ||
H1-LF | TCTCCCTTCTTGATCCC | 10 μM | 713–729 | 17 | ||
H1-LB | TAGTGGTACCGAGATATGCA | 10 μM | 794–813 | 20 | ||
A/H3 | HA | H3-F3 | GGGGTTACTTCAAAATACAAG | 5 μM | 841–859 | 19 |
H3-B3 | GTTGCCAATTTCAGAGTGTT | 5 μM | 1011–1028 | 18 | ||
H3-FIP | GAGTGATGCATTCAGAATTGCATTT-TGGGAAAAGCTCAATAATGAGA | 40 μM | 903–927 + 863–884 | 47 | ||
H3-BIP | AATGGAAGCATTCCCAATGACA-GCTTAACATATCTGGGACAGG | 40 μM | 930–951 + 988–1008 | 43 | ||
H3-LF | CCAATGGGTGCATCTGA | 10 μM | 885–901 | 17 | ||
H3-LB | AACCATTCCAAAATGTAAAC | 10 μM | 952–971 | 20 | ||
A/H5 | HA | H5-F3 | GCTATAGCAGGTTTTATAGAGG | 5 μM | 1048–1069 | 22 |
H5-B3 | GCCTCAAACTGAGTGTTCAT | 5 μM | 1210–1229 | 20 | ||
H5-FIP | ACTCCCCTGCTCATTGCTAT-GGATGGCAGGGAATGGTA | 80 μM | 1112–1131 + 1072–1089 | 38 | ||
H5-BIP | GGTACGCTGCAGACAAAGAAT-TGAGTTGACCTTATTGGTGAC | 80 μM | 1133–1153 + 1177–1197 | 42 | ||
H5-LF | GGTACCCATACCAACCATC | 5 μM | 1093–1114 | 19 | ||
H5-LB | CCACTCAAAAGGCAATAGATGGA | 5 μM | 1154–1176 | 23 | ||
A/H7 | HA | H7-F3 | GCGGGTTTCATTGAAAATGG | 5 μM | 1036–1055 | 20 |
H7-B3 | CTACCTCATTGAATTCATTGTCT | 5 μM | 1215–1237 | 23 | ||
H7-FIP | TCCCTCTCCCTGTGCATTCT-ATGGGAAGGCCTAATTGATG | 80 μM | 1097–1116 + 1056–1075 | 40 | ||
H7-BIP | ACTGCTGCAGATTACAAAAGCAC-TGGTTGGTTTTTTCTATAAGCC | 80 μM | 1117–1139 + 1178–1199 | 45 | ||
H7-LF | TCTGAAACCATACCAAC | 5 μM | 1076–1092 | 17 | ||
H7-LB | TCAATCGGCAATTGATCAAATA | 5 μM | 1140–1161 | 22 |