Table 1 Nested RT-PCR and real time RT-PCR primers and probe used in this study
Target gene | PCR | Primer name | Sequences | Product size (bp) |
|---|---|---|---|---|
M segment | Nested RT-PCR (1st PCR) | SFTS-M 1st-F | TCATCCTGACYTATTYTGCAATWG | 640 |
SFTS-M 1st-R | TAAGTYACACTCACACCCTTGAA | |||
Nested RT-PCR (2nd PCR) | SFTS-F (=MF3) | GATGAGATGGTCCATGCTGATTCTAA | 560 | |
SFTS-R (=MR2) | CTCATGGGGTGGAATGTCCTCAC | |||
S segment | Real-time RT-PCR | SFTS-SQ-F | ACCTCTTTGACCCTGAGTTWGACA | 120 |
SFTS-SQ-R | CTGAAGGAGACAGGTGGAGATGA | |||
SFTS-SQ-P | [FAM]-TGCCTTGACGATCTTA [NFQ-MGB] |