Table 4 Comparisons of mutations found in erm(41) and rrl for each subspecies of the M. abscessus using sequencing and real-time PCR
Phenotypic Result | Sequencing Results | Real-time PCR Results | ||||||
|---|---|---|---|---|---|---|---|---|
Subspecies | # of Samples | Full-length/truncated erm41 | erm41 position 28 | rrl position 2058 | Full-length/truncated erm41 | erm41 position 28 | rrl position 2058 | |
M. abscessus subsp. abscessus | ||||||||
67 | IR | Full | T | A | Full | T | A | |
1 | Ra | Full | T | A | Full | T | A | |
1 | Sa | Full | T | A | Full | T | A | |
1 | R | Full | T | C | Full | T | C | |
21 | S | Full | C | A | Full | C | A | |
2 | IR | Full | C | A | Full | T + C | A | |
1 | IRa | Full | C | A | Full | C | A | |
M. abscessus subsp. bolletii | ||||||||
5 | IR | Full | T | A | Full | T | A | |
2 | S | Full | C | A | Full | C | A | |
M. abscessus subsp. massiliense | ||||||||
21 | S | Truncated | T | A | Truncated | T | A | |
1 | R | Truncated | T | C | Truncated | T | C | |
1 | IR | Full | T | A | Full | T | A | |
b M. abscessus | ||||||||
1 | S | Full | C | A | Full | C | A | |
1 | S | Truncated | T | A | Full | C | A | |