Table 2 Probes and primers used in the real-time assay for M. abscessus
Target | Sequence | Reference | |
|---|---|---|---|
16S | Absc-chel 16S | 5′-6FAM-ACC ACA CAC TTC A-MGB-NFQ-3’ | [16] |
F Primer16S | 5′-ATAAGCCTGGGAAACTGGGTCTA-3’ | ||
R Primer16S | 5′-CCACACCGCAAAAGCTTT-3’ | ||
Full length erm(41) | erm(41)_probe1 | 5′-6FAM-TGC TAG CCG TCG AGC TGC ATC C-QSY-3’ | [17] |
F PrimerTR | 5′-TCAGGGGAGTTCGTTGTGGAT-3’ | ||
R PrimerTR | 5′-TCTTCCTCGGCAAACCGT-3’ | ||
erm(41) | erm(41)T28 | 5′-HEX-CCA + G + T + G GGG C-IABkFQ-3’ | [12] |
erm(41)C28 | 5′-6FAM-CCA + G + C + G GGGC-IABkFQ-3’ | ||
F PrimerE28 | 5′-GAGCATGGGCATATTCATGATGG-3’ | ||
R PrimerE28 | 5′-TGAGCGAACACCGGATTCG-3’ | ||
rrl | 23S_A2058 | 5′-6FAM-CGGCAGGACGAAAAGACCC-BHQ1–3’ | [12] |
23S_C2058 | 5′-6FAM-CGGCAGGACGACAAGACCC-BHQ1–3’ | ||
F PrimerR2058 | 5′-GCGAAATTGCACTACGAGTAAAG-3’ | ||
R PrimerR2058 | 5′-CCTATCCTACACAAACCGAACC-3’ | ||