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Table 3 Galactomannan (GM), β-D-glucan and glucuronoxylomannan antigen assays on culture supernatant. For each strain, 5 to 10 colonies incubated at 35 °C for 4 days on Sabouraud media were suspended in 1 ml distilled water. After vigorous agitation, the suspensions were centrifuged for 5 min at 10,000 g. 1, 1:10 and 1:100 dilutions of the supernatants were then tested with Platelia® Aspergillus assay (Bio-Rad, France), Fungitell® assay (Associates of Cape Cod Inc., USA) and Biosynex® CryptoPS assay (Biosynex, France) according to the manufacturer’s recommendations

From: Hormographiella aspergillata: an emerging basidiomycete in the clinical setting? A case report and literature review

Isolate

Dilution factor

Galactomannan

β-D-glucan (pg/mL)

Glucuronoxylomannan

HA2

1

> 3,5

> 500

Negative

10

> 3,5

> 500

n.d.

100

0,2423

51,048

n.d.

HA3

1

> 3,5

> 500

Negative

10

> 3,5

> 500

n.d.

100

0,4197

98,804

n.d.

  1. n.d. Not determined