Table 1 PCR primers used in the study
Primer | Primers (5_ to 3_) | Amplicon size (bp) | Annealing temperature °C | Reference |
|---|---|---|---|---|
pCVD432 | F-CTGGCGAAAGACTGTATCAT R-CAATGTATAGAAATCCGCTGTT | 630 | 58 | [19] |
eae | F-CTGAACGGCGATTACGCGAA R-CCAGACGATACGATCCAG | 917 | 58 | [20] |
bfpA | F-AATGGTGCTTGCGCTTGCTGC R-GCCGCTTTATCCAACCTGGTA | 326 | 58 | [21] |
LT gene | F-GGCGACAGATTATACCGTGC R-CGGTCTCTATATTCCCTGTT | 450 | 50 | [22] |
ST gene | F-ATTTTTMTTTCTGTATTRTCTT R-CACCCGGTACARGCAGGATT | 190 | 50 | [22] |
ipaH | F-GTTCCTTGACCGCCTTTCCGATACCGTC R-GCCGGTCAGCCACCCTCTGAGAGTAC | 600 | 52 | [23] |
stx1 | F-ATAAATCGCCATTCGTTGACTAC R-AGAACGCCCACTGAGATCATC | 180 | 50 | [24] |
Stx2 | F-GGCACTGTCTGAAACTGCTCC R-TCGCCAGTTATCTGACATTCTG | 255 | 50 | [22] |
ChuA | F- ACGAACCAACGGTCAGGAT R-TGCCGCCAGTACCAAAGACA | 279 | 59 | [10] |
yjaA | F-TGAAGTGTCAGGAGACGCTG R-ATGGAGAATGCGTTCCTCAAC | 211 | 59 | [10] |
TspE4C2 | F-GAGTAATGTCGGGGCATTCA R-CGCGCCAACAAAGTATTACG | 154 | 59 | [10] |
blaTEM | F-AAACGCTGGTGAAAGTA R-AGCGATCTGTCTAT | 822 | 58 | [25] |
blaSHV | F-ATGCGTTATATTCGCCTGTG R-TGCTTTGTTATTCGGGCCAA | 753 | 60 | [25] |
CTX-M-15 | F-CACACGTGGAATTTAGGGACT R-GCCGTCTAAGGCCATAAACA | 996 | 55 | [26] |
MOX | F-GCTGCTCAAGGAGCACAGGAT R-CAC ATT GAC ATA GGT GTG GTG C | 520 | ||
FOX | F-AAC ATG GGG TAT CAG GGA GAT G R-CAA AGC GCG TAA CCG GAT TGG | 190 | 64 | [27] |
DHA | F-AAC TTT CAC AGG TGT GCT GGG T R-CCG TAC GCA TAC TGG CTT TGC | 405 | ||
CIT | F-TGG CCA GAA CTG ACA GGC AAA R-TTT CTC CTG AAC GTG GCT GGC | 462 |