Skip to main content

Table 1 PCR primers used in the study

From: Prevalence, phylogeny, and antimicrobial resistance of Escherichia coli pathotypes isolated from children less than 5 years old with community acquired- diarrhea in Upper Egypt

Primer Primers (5_ to 3_) Amplicon
size (bp)
Annealing temperature °C Reference
pCVD432 F-CTGGCGAAAGACTGTATCAT
R-CAATGTATAGAAATCCGCTGTT
630 58 [19]
eae F-CTGAACGGCGATTACGCGAA
R-CCAGACGATACGATCCAG
917 58 [20]
bfpA F-AATGGTGCTTGCGCTTGCTGC
R-GCCGCTTTATCCAACCTGGTA
326 58 [21]
LT gene F-GGCGACAGATTATACCGTGC
R-CGGTCTCTATATTCCCTGTT
450 50 [22]
ST gene F-ATTTTTMTTTCTGTATTRTCTT
R-CACCCGGTACARGCAGGATT
190 50 [22]
ipaH F-GTTCCTTGACCGCCTTTCCGATACCGTC
R-GCCGGTCAGCCACCCTCTGAGAGTAC
600 52 [23]
stx1 F-ATAAATCGCCATTCGTTGACTAC
R-AGAACGCCCACTGAGATCATC
180 50 [24]
Stx2 F-GGCACTGTCTGAAACTGCTCC
R-TCGCCAGTTATCTGACATTCTG
255 50 [22]
ChuA F- ACGAACCAACGGTCAGGAT
R-TGCCGCCAGTACCAAAGACA
279 59 [10]
yjaA F-TGAAGTGTCAGGAGACGCTG
R-ATGGAGAATGCGTTCCTCAAC
211 59 [10]
TspE4C2 F-GAGTAATGTCGGGGCATTCA
R-CGCGCCAACAAAGTATTACG
154 59 [10]
blaTEM F-AAACGCTGGTGAAAGTA
R-AGCGATCTGTCTAT
822 58 [25]
blaSHV F-ATGCGTTATATTCGCCTGTG
R-TGCTTTGTTATTCGGGCCAA
753 60 [25]
CTX-M-15 F-CACACGTGGAATTTAGGGACT
R-GCCGTCTAAGGCCATAAACA
996 55 [26]
MOX F-GCTGCTCAAGGAGCACAGGAT
R-CAC ATT GAC ATA GGT GTG GTG C
520   
FOX F-AAC ATG GGG TAT CAG GGA GAT G
R-CAA AGC GCG TAA CCG GAT TGG
190 64 [27]
DHA F-AAC TTT CAC AGG TGT GCT GGG T
R-CCG TAC GCA TAC TGG CTT TGC
405   
CIT F-TGG CCA GAA CTG ACA GGC AAA
R-TTT CTC CTG AAC GTG GCT GGC
462