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Fig. 3 | BMC Infectious Diseases

Fig. 3

From: Potential anti-EBV effects associated with elevated interleukin-21 levels: a case report

Fig. 3

Frequencies of CD4+ IL-21+ T cells and pTFH prior to and after vaccination. PBMC cultures (1 × 106/mL), sampled pre-, seven and 21 days post-vaccination, were stimulated for three hours with PMA (20 ng/mL) and ionomycin (300 ng/mL). Staining for surface CD4+ and intra-cellular IL-21 was performed on PBMC derived from the patient a and three (2 of whom were age matched) female controls b (patient vs. controls (all time points), p = 0.01, Mann- Whitney U test). Frequencies of CD4+ CD45RA CXCR5+ CCR7low PD-1high p (eripheral) TFH were ascertained ex-vivo, using the gating strategy devised by He et al. [8], in PBMC (1 × 106/mL), sampled pre-, seven and 21 days post-vaccination. Frequencies of CCR7low and PD-1high expression among patient c and controls d CD4+ CD45RA CXCR5+ T cells are shown (patient vs. controls (all time points), p = 0.01, Mann-Whitney U test). Median and minimum-maximum values are shown. The dot-plots for the PE-conjugated isotype control (mouse IgG1K) antibody in PMA and inonomycin stimulated CD4+T cells from e the patient and f a control. FI: fluorescence intensity, PE: phycoerythrin, BV: Brilliant Violet, PerCP: Peridinin-Chlorophyll-Protein

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