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Fig. 2 | BMC Infectious Diseases

Fig. 2

From: Bone marrow-derived mesenchymal stem cells attenuate pulmonary inflammation and lung damage caused by highly pathogenic avian influenza A/H5N1 virus in BALB/c mice

Fig. 2

MSCs culture and identification. MSCs were isolated from bone marrow of BALB/c mice as described in Materials and Methods. The cells were suspended in growth medium α (Gibco, USA) and cultured in petri dishes at 37 °C in a 5% CO2 incubator. Micrographs of cultured cells on day 11 are shown in a with a X100 and b with a X400 magnification, and on day 28 in c with a X400 magnification. Spindle- or oval-shaped cells like as indicated by a yellow arrow in b are MSCs, while round shaped cells like as indicated by a light blue arrow are hematopoietic stem cells which disappeared on day 28. For confirmation of MSCs, the isolated MSCs cells were incubated for 1 h to attach to petri dish and subjected to immunocytochemical analysis. d Flow cytometry analysis of the cultured MSCs. Red scattered spots show CD105-positive and CD45-negative cells indicating 99.9% of total cells were MSCs. e In the staining with anti-CD105, all of the cells appeared to glow greenish fluorescence under a X100 magnification, indicating that they were positive for CD105. f In the staining with anti-CD45, greenish fluorescence was absent, indicating that they were negative for CD45. g The ant-CD45 positively stained hematopoietic stem cells provided by Stem Cell Research and Development Center, Airlangga University as a staining-positive control

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