From: Evaluation of the performance of an in-house duplex PCR assay targeting the IS6110 and rpoB genes for tuberculosis diagnosis in Cameroon
Gene (PCR product size bp)
Primer name
Primer sequence (5′- 3′)
Annealing temperature
rpoB (235)
rpoBF
TACGGTCGGCGAGCTGATCCAAA
68 °C
rpoBR
ACAGTCGGCGCTTGTGGGTCAAC
IS6110 (123)
IS6110F
CCTGCGAGCGTAGGCGTCGG
63 °C
IS6110R
CTCGTCCAGCGCCGCTTCGG