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Table 1 Primers used and their sequences

From: Evaluation of the performance of an in-house duplex PCR assay targeting the IS6110 and rpoB genes for tuberculosis diagnosis in Cameroon

Gene (PCR product size bp)

Primer name

Primer sequence (5′- 3′)

Annealing temperature

rpoB (235)

rpoBF

TACGGTCGGCGAGCTGATCCAAA

68 °C

rpoBR

ACAGTCGGCGCTTGTGGGTCAAC

 

IS6110 (123)

IS6110F

CCTGCGAGCGTAGGCGTCGG

63 °C

IS6110R

CTCGTCCAGCGCCGCTTCGG