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Table 6 Performance characteristics of the three tests in Melong HD. Summary of sensitivity, specificity, PPV and NPV for Microscopy, actin-qPCR and O-150 LAMP at different time points following direct observed treatment of microfilaridermic individuals with ivermectin in Melong Health District

From: Differential susceptibility of Onchocerca volvulus microfilaria to ivermectin in two areas of contrasting history of mass drug administration in Cameroon: relevance of microscopy and molecular techniques for the monitoring of skin microfilarial repopulation within six months of direct observed treatment

METHODS SENSITIVITY SPECIFICITY PPV NPV
D30
 MICROSCOPY 45.4 (10.1–91.2) 99.1 (89.7–100) 89.6 (29.3–100) 91.1 (71.0–99.2)
 REAL-TIME PCR 87.6 (32.2–100) 98.7 (87.1–100) 92.2 (36.2–100) 98.0 (78.3–100)
 LAMP ASSAY 94.2 (52.0–100) 89.3 (71.5–99.7) 60.5 (18.4–99.3) 98.9 (87.4–100)
D90
 MICROSCOPY 90.6 (48.3–100) 94.7 (82.3–100) 78.9 (35.2–99.9) 98.0 (82.0–100)
 REAL-TIME PCR 38.0 (10.9–80.6) 99.4 (92.9–100) 93.1 (43.4–100) 87.8 (69.8–97.9)
 LAMP ASSAY 92.1 (55.3–100) 89.8 (75.9–99.7) 66.1 (28.1–99.2) 98.1 (84.9–100)
D180
 MICROSCOPY 88.9 (58.1–99.9) 96.1 (84.9–99.8) 87.5 (59.2–99.4) 96.5 (84.5–100)
 REAL-TIME PCR 96.7 (69.9–100) 98.8 (89.2–100) 96.2 (66.7–100) 99.0 (89.4–100)
 LAMP ASSAY 97.1 (74.0–100) 75.3 (58.7–88.5) 55.0 (30.6–78.2) 98.9 (87.7–100)
D30 + D90 + D180
 MICROSCOPY 80.2 (57.2–95.0) 95.9 (89.4–99.2) 81.5 (56.6–96.4) 95.7 (87.9–99.0)
 REAL-TIME PCR 78.3 (53.3–97.9) 99.7 (96.5–100) 98.2 (82.1–100) 95.4 (87.4–99.7)
 LAMP ASSAY 98.5 (84.3–100) 84.7 (75.6–92.3) 58.7 (39.1–79.4) 99.6 (95.4–100)