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Table 5 Performance characteristics of the three tests in Bafia HD. Summary of sensitivity, specificity, PPV and NPV for microscopy, actin-qPCR and O-150 LAMP at different time points following direct observed treatment of microfilaridermic individuals with ivermectin in Bafia health district

From: Differential susceptibility of Onchocerca volvulus microfilaria to ivermectin in two areas of contrasting history of mass drug administration in Cameroon: relevance of microscopy and molecular techniques for the monitoring of skin microfilarial repopulation within six months of direct observed treatment

METHODS SENSITIVITY SPECIFICITY PPV NPV
D30
 MICROSCOPY 89.6 (61.6–99.9) 99.3 (93.0–100) 97.6 (76.9–100) 96.9 (86.2–100)
 REAL-TIME PCR 89.6 (64.5–99.6) 96.8 (87.5–99.9) 89.6 (62.2–99.7) 96.8 (87.2–99.9)
 LAMP ASSAY 97.6 (78.7–100) 92.1 (80.6–98.8) 79.0 (53.2–97.1) 99.2 (91.9–100)
D90
 MICROSCOPY 71.4 (45.8–91.8) 96.9 (88.0–99.8) 90.6 (67.0–99.5) 88.8 (74.8–97.3)
 REAL-TIME PCR 97.5 (75.8–100) 97.0 (86.3–100) 93.2 (69.3–99.9) 98.9 (87.8–100)
 LAMP ASSAY 97.5 (79.0–100) 88.7 (75.0–97.6) 78.6 (54.5–95.7) 98.8 (89.1–100)
D180
 MICROSCOPY 80.6 (62.9–93.1) 94.8 (81.3–99.6) 94.9 (80.6–99.6) 80.8 (61.5–93.4)
 REAL-TIME PCR 98.9 (87.3–100) 98.5 (84.6–100) 98.8 (86.4–100) 98.7 (83.4–100)
 LAMP ASSAY 99.0 (90.1–100) 76.6 (56.3–91.6) 83.4 (66.9–94.3) 98.6 (85.2–100)
D30 + D90 + D180
 MICROSCOPY 80.7 (68.4–90.2) 97.4 (93.1–99.5) 94.5 (85.1–98.9) 90.3 (83.1–95.2)
 REAL-TIME PCR 98.1 (90.0–100) 97.6 (92.7–99.8) 95.7 (87.1–99.6) 99.0 (94.2–100)
 LAMP ASSAY 99.4 (93.4–100) 87.1 (79.4–93.1) 80.7 (69.6–89.9) 99.6 (95.8–100)