Differential susceptibility of Onchocerca volvulus microfilaria to ivermectin in two areas of contrasting history of mass drug administration in Cameroon: relevance of microscopy and molecular techniques for the monitoring of skin microfilarial repopulation within six months of direct observed treatment

Table 4 Microfilaridemia detection rate by microscopy, actin-qPCR and O-150 LAMP at day 30, 60 and 180 post IVM treatment in Melong HD

Time point after treatment (days)	Number of consented eligible participants	Number of samples collected	Proportion positive for microscopy n(%)	Proportion positive for real-time PCR n(%)	Proportion positive for O-150 LAMP n(%)
D30	44	29	2 (6.9%)	4 (13.8%)	7 (24.1%)
D90	44	44	9 (20.5%)	7 (15.9%)	12 (27.3%)
D180	44	38	9 (23.7%)	9 (23.7%)	16 (42.1%)
Total		111	20 (18.0%)	20 (18.0%)	35 (31.5%)
Difference			P = 0.180	P = 0.520	P = 0. 215

ISSN: 1471-2334