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Fig. 1 | BMC Infectious Diseases

Fig. 1

From: Evaluation of toxoplasmosis in pregnant women using dot-immunogold-silver staining with recombinant Toxoplasma gondii peroxiredoxin protein

Fig. 1

Detection of rTgPrx by SDS-PAGE and Western blotting. a: rTgPrx was expressed in E. coli, purified using GST affinity chromatography, analyzed by SDS-PAGE on 12% gels, and stained with Coomassie brilliant blue. M: marker. Lane 1: The molecular weight of rTgPrx digested by PreScission Protease was 25‚ÄČkDa. Lane 2: GST-tagged rTgPrx was purified by GST affinity chromatography and had a molecular weight of 51‚ÄČkDa. Lane 3: SDS-PAGE showed that unpurified rTgPrx was expressed in E. coli. b: The immunoreactivity of rTgPrx was detected by Western blotting. M: marker. Lane 1: rTgPrx was reacted with rabbit anti-T.gondii serum. Lane 2: rTgPrx was reacted with negative serum

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