Fig. 3
From: Case report: multiple and atypical amoebic cerebral abscesses resistant to treatment
Immunodetection of E. histolytica trophozoites in brain tissue by immunohistochemistry and immunofluorescence. Identification of the E. histolytica 140 kDa fibronectin (FN)-binding protein (EhFNR) and glial fibrillary acidic protein (GFAP) in brain tissue by immunofluorescence. a Amoebic trophozoites stained using peroxidase-labeled rabbit anti-E. histolytica polyclonal antibody (X 1000). b E. histolytica actin cytoskeleton dynamics and distribution in amebic brain abscesses. Actin was stained with rhodamine-phalloidin (1:40, red), forming plate adhesions, as shown by the yellow arrow (X 400). c E. histolytica trophozoites stained positive for EhFNR (red), GFAP (green) and nuclei (Hoechst 1:1000, blue) in amebic brain abscess tissue (X400). d GFAP-immunoreactive cells in brain sections (green) and nuclei (blue) (X400)