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Table 1 Summary of MinION data from all reference samples sequenced with name, subtype and barcode. Mean coverage, mapped reads and number of nucleotide differences are shown for each individual segment. The consensus identity is calculated for all segments in %

From: Rapid multiplex MinION nanopore sequencing workflow for Influenza A viruses

Barcode Sample Subtype   PB2 PB1 PA HA NA NP MP NS Sum Consensus identity %
1 R30–06 H1N1 Mean Coverage 749.9 188.3 700.9 2771.9 8756.7 7987.3 13,551.5 11,637.7 5793.025 99.98528113
Mapped Reads 8102 1131 4059 8207 22,156 20,995 31,635 30,095 126,380
Nucleotide Differences     1 1     2
2 R3111–07 H2N9 Mean Coverage 2250.2 1360.5 1001 4396.1 626.7 4582.6 8212.6 7477 3738.3375 99.99264057
Mapped Reads 32,171 11,283 7372 11,560 2390 10,367 18,613 18,298 112,054
Nucleotide Differences     1      1
3 R2555–06 H3N1 Mean Coverage 6373.3 2200.9 2080.6 929.1 1968 3874.8 5749.1 5016.4 3524.025 99.98528113
Mapped Reads 40,737 14,924 14,166 4074 6248 9628 13,859 8970 112,606
Nucleotide Differences   1    1     2
4 HAIV-81 H4N6 Mean Coverage 1462 3401.4 792.2 4161.2 8039.7 11,356.3 14,790.1 9955.9 6744.85 99.98528113
Mapped Reads 18,920 20,238 6874 11,778 20,138 27,900 35,597 23,680 165,125
Nucleotide Differences 1    1      2
5 R1612–08 H5N3 Mean Coverage 5033.4 3013.7 5260.7 1441.3 3776.2 1802.1 6314.7 5898.6 4067.5875 99.96320283
Mapped Reads 35,383 19,354 31,659 8876 10,321 4821 14,220 12,908 137,542
Nucleotide Differences 1 1   2 1     5
6 R617–07 H6N2 Mean Coverage 3933.5 2889.7 2995.6 4024 3403.6 3564.3 9820.9 5480.4 4514 99.99264057
Mapped Reads 29,256 21,688 23,359 13,971 9179 8292 22,581 13,515 141,841
Nucleotide Differences     1      1
7 R11–01 H7N7 Mean Coverage 5558.2 1201 1696.6 3737.4 3467.6 6623.9 7390.9 6832.7 4563.5375 99.98528113
Mapped Reads 46,729 12,888 17,912 15,132 8502 16,267 18,781 16,333 152,544
Nucleotide Differences   1 1       2
8 R249–08 H9N2 Mean Coverage 4445.5 3087.6 2260.4 1729.2 2652.5 2341.6 5403.8 6044.2 3495.6 99.98528113
Mapped Reads 32,149 20,998 14,076 6316 7275 6366 12,914 13,244 113,338
Nucleotide Differences    1 1      2
9 WV1677–03 H10N4 Mean Coverage 4296.7 1296.4 2896.6 3175.4 2728 3976.2 7689.3 5656 3964.325 99.9779217
Mapped Reads 41,559 12,764 26,781 9445 8429 10,422 17,043 13,420 139,863
Nucleotide Differences    2   1     3
10 R2675–06 H11N6 Mean Coverage 924.2 912.9 1378.1 1499.1 2260.8 4951.9 4345.1 3944 2527.0125 99.99264057
Mapped Reads 14,361 12,470 13,834 5702 5402 12,233 10,004 9893 83,899
Nucleotide Differences 1         1
11 R2613–06 H13N8 Mean Coverage 866.9 1040.9 1199.3 6152 5035.8 10,790.8 19,984.4 16,654.6 7715.5875 99.9779217
Mapped Reads 9250 10,656 10,600 19,030 14,503 26,581 48,937 44,445 184,002
Nucleotide Differences 1 1    1     3
12 Se-99 H16N3 Mean Coverage 9922 426.7 599.1 115.8 2673.5 2096 5058.4 5190.9 3260.3 99.96320283
Mapped Reads 3843 2695 5041 393 11,992 5296 12,593 11,951 53,804
Nucleotide Differences 1 1   1 2     5