Fig. 6
Identification of env-defective gene after 20th passage. The env-defective sequences G20R1 and G21R1 as well as the NFLG sequence FL-21a were aligned with the sequence of SG3 and SG3Δenv. The characteristic of the 4-nucleoid acid (AGCT) insertion after position 423 T was identified in the sequences isolated from the 20th and 21st passages in the host cell genome. Position numbering is relative to the env of SG3