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Table 4 Inter-assay variation in multiplex detection of H5 and N8 avian influenza viruses

From: Development and evaluation of a TaqMan MGB RT-PCR assay for detection of H5 and N8 subtype influenza virus

Plasmid copy number

(per reaction)

Gene

Ct value in intra-assays

Mean ± SD

CV%

Day 1

Day 3

Day 5

1 × 105

H5

23.97

24.36

23.53

23.95 ± 0.34

1.42%

N8

22.83

22.43

23.06

22.77 ± 0.26

1.14%

1 × 104

H5

26.60

27.01

27.32

26.98 ± 0.29

1.09%

N8

26.58

27.01

26.45

26.68 ± 0.24

0.90%

1 × 103

H5

32.46

32.12

31.75

32.11 ± 0.29

0.90%

N8

30.69

30.38

30.56

30.54 ± 0.13

0.42%

1 × 102

H5

34.04

34.20

34.68

34.31 ± 0.27

0.79%

N8

34.05

35.41

34.55

34.67 ± 0.56

1.62%

1 × 101

H5

38.91

38.59

38.23

38.58 ± 0.28

0.72%

N8

38.46

38.00

38.35

38.27 ± 0.20

0.51%

1 × 100

H5

No Ct

No Ct

No Ct

  

N8

No Ct

No Ct

No Ct

  
  1. Note: We repeated multiplex RT-PCR on days 1, 3 and 5. CV% (threshold = 3%) and Ct values for each concentration are shown