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Table 4 Inter-assay variation in multiplex detection of H5 and N8 avian influenza viruses

From: Development and evaluation of a TaqMan MGB RT-PCR assay for detection of H5 and N8 subtype influenza virus

Plasmid copy number
(per reaction)
Gene Ct value in intra-assays Mean ± SD CV%
Day 1 Day 3 Day 5
1 × 105 H5 23.97 24.36 23.53 23.95 ± 0.34 1.42%
N8 22.83 22.43 23.06 22.77 ± 0.26 1.14%
1 × 104 H5 26.60 27.01 27.32 26.98 ± 0.29 1.09%
N8 26.58 27.01 26.45 26.68 ± 0.24 0.90%
1 × 103 H5 32.46 32.12 31.75 32.11 ± 0.29 0.90%
N8 30.69 30.38 30.56 30.54 ± 0.13 0.42%
1 × 102 H5 34.04 34.20 34.68 34.31 ± 0.27 0.79%
N8 34.05 35.41 34.55 34.67 ± 0.56 1.62%
1 × 101 H5 38.91 38.59 38.23 38.58 ± 0.28 0.72%
N8 38.46 38.00 38.35 38.27 ± 0.20 0.51%
1 × 100 H5 No Ct No Ct No Ct   
N8 No Ct No Ct No Ct   
  1. Note: We repeated multiplex RT-PCR on days 1, 3 and 5. CV% (threshold = 3%) and Ct values for each concentration are shown