Plasmid copy number (per reaction) | Gene | Ct value in intra-assays | Mean ± SD | CV% | ||
---|---|---|---|---|---|---|
Day 1 | Day 3 | Day 5 | ||||
1 × 105 | H5 | 23.97 | 24.36 | 23.53 | 23.95 ± 0.34 | 1.42% |
N8 | 22.83 | 22.43 | 23.06 | 22.77 ± 0.26 | 1.14% | |
1 × 104 | H5 | 26.60 | 27.01 | 27.32 | 26.98 ± 0.29 | 1.09% |
N8 | 26.58 | 27.01 | 26.45 | 26.68 ± 0.24 | 0.90% | |
1 × 103 | H5 | 32.46 | 32.12 | 31.75 | 32.11 ± 0.29 | 0.90% |
N8 | 30.69 | 30.38 | 30.56 | 30.54 ± 0.13 | 0.42% | |
1 × 102 | H5 | 34.04 | 34.20 | 34.68 | 34.31 ± 0.27 | 0.79% |
N8 | 34.05 | 35.41 | 34.55 | 34.67 ± 0.56 | 1.62% | |
1 × 101 | H5 | 38.91 | 38.59 | 38.23 | 38.58 ± 0.28 | 0.72% |
N8 | 38.46 | 38.00 | 38.35 | 38.27 ± 0.20 | 0.51% | |
1 × 100 | H5 | No Ct | No Ct | No Ct |  |  |
N8 | No Ct | No Ct | No Ct | Â | Â |