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Table 3 Intra-assay variation in multiplex detection of H5 and N8 avian influenza viruses

From: Development and evaluation of a TaqMan MGB RT-PCR assay for detection of H5 and N8 subtype influenza virus

Plasmid copy number
(per reaction)
Gene Ct value in intra-assays Mean ± SD CV%
1 2 3
1 × 105 H5 24.36 24.37 24.30 24.34 ± 0.03 0.13%
N8 23.97 23.72 23.97 23.89 ± 0.12 0.49%
1 × 104 H5 28.73 28.88 29.07 28.89 ± 0.14 0.48%
N8 26.97 27.04 27.02 27.01 ± 0.03 0.11%
1 × 103 H5 32.01 32.12 32.00 32.04 ± 0.05 0.17%
N8 30.01 30.23 30.14 30.13 ± 0.09 0.30%
1 × 102 H5 34.79 34.51 34.68 34.66 ± 0.12 0.33%
N8 34.05 34.44 34.41 34.30 ± 0.18 0.52%
1 × 101 H5 38.59 38.23 38.13 38.32 ± 0.20 0.52%
N8 37.86 37.77 37.87 37.83 ± 0.04 0.12%
1 × 100 H5 No Ct No Ct No Ct   
N8 No Ct No Ct No Ct   
  1. Note: Each concentration included three replicates on one plate. CV% (threshold = 3%) and Ct values for each concentration are shown