Fig. 1

Amplification plots and standard curves of H5 (A and B) and N8 (C and D) assays. HA and NA gene sequences of pHW2000-H5 and pHW2000-N8 are from A/duck/Zhejiang/W24/ 2013(H5N8), an H5N8 AIV of clade 2.3.4.4 isolated in 2013