Fig. 3

Amplification curves of herpesviruses quantitative real-time polymerase-chain reaction assays. On the x-axis the cycle numbers are represented. On the y-axis the fluorescence signals are reported on a logarithmic scale; Rn is the fluorescence of the reporter dye divided by the fluorescence of the passive reference dye. Green lines represent the four internal controls. The other four curves represent the viral targets as indicated in the Figure (i.e. VZV, CMV, EBV, and HHV-6). The horizontal central line is the threshold line