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Table 1 Laboratory specific susceptibility testing parameters and interpretation standards used in participating laboratories

From: External quality assessment (EQA) of Neisseria gonorrhoeae antimicrobial susceptibility testing in primary laboratories in Germany

 

No. of labs

Test interpretation according to guidelines

 EUCAST

19

 mixeda

2

Type of susceptibility test used

 MIC gradient strips

20

 MIC gradient strips/disc diffusionb

1

 Agar dilutionc

1

MIC gradient strip manufacturer

 Biomerieux

4

 Liofilchem

10

 Oxoid

1

 mixedd

1

 no information provided

5

Agar base

 GC chocolatised blood agar

9

 Mueller-Hinton chocolatised blood agar

3

 Mueller-Hinton fastidious (MH-F)

6

 Selective agar

2

 No clear information provided

1

Incubation CO2 concentration

 3%

2

 5%

19

Incubation temperature

 35 °C

6

 36 °C

13

 37 °C

2

Incubation humidity

 50%

4

 60%

2

 70%

4

 80%

1

 90%

7

 100%

3

Incubation time until MIC reading

 16 h

1

 19 h

1

 20 h

5

 22 h

2

 24 h

12

  1. For azithromycin: EUCAST (1 lab), unknown (1 lab)
  2. aCLSI for all antibiotics except for azithromycin
  3. bdisc diffusion for azithromycin, penicillin, ciprofloxacin
  4. cin addition to Etest
  5. dLiofilchem exept for ceftriaxone (Biomerieux)