Table 1 Primer sequences in this study
Gene | Locus | Round | Primers | Sequence (5′ → 3′) | Amplicons (bp) |
|---|---|---|---|---|---|
pfcrt [27] | 74,75,76 | 1st | CRT1–1 | CCGTTAATAATAAATACACGCAG | 537 |
CRT1–2 | CGGATGTTACAAAACTATAGTTACC | ||||
2nd | CRT2–1 | TGTGCTCATGTGTTTAAACTT | 145 | ||
CRT2–2 | CAAAACTATAGTTACCAATTTTG | ||||
pfmdr1 [29] | 86,184 | 1st | MDR1–1F | TTAAATGTTTACCTGCACAACATAGAAAATT | 612 |
MDR1–1R | CTCCACAATAACTTGCAACAGTTCTTA | ||||
2nd | MDR1–2F | TGTATGTGCTGTATTATCAGGA | 526 | ||
MDR1–2R | CTCTTCTATAATGGACATGGTA | ||||
1034, 1042 | 1st | 1042-A | GTCGAAAAGACTATGAAACGTAGA | 350 | |
1042-C | CTCAAATGATAATTTTGCAT | ||||
2nd | 1042-B | GATCCAAGTTTTTTAATACA | |||
1042-C | CTCAAATGATAATTTTGCAT | ||||
1246 | 1st | 1246-A | GTGGAAAATCAACTTTTATGA | 410 | |
1246-B | TTAGGTTCTCTTAATAATGCT | ||||
2nd | 1246-C | GACTTGAAAAATGATCACATT | |||
1246-D | GTCCACCTGATATGCTTTT | ||||
K13-propeller [30] |  | 1st | K13–1-1 | CGGAGTGACCAAATCTGGGA | 2063 |
K13–1-2 | GGGAATCTGGTGGTAACAGC | ||||
2nd | K13–2-1 | GCCTTGTTGAAAGAAGCAGA | 849 | ||
K13–2-2 | GCCAAGCTGCCATTCATTTG |