Table 1 Primer sets for amplification of the whole genome of WUPyV
From: Isolation and characterization of WUPyV in polarized human airway epithelial cells
Primers | Primer sequence (5′-3′) | Location (nt)a | Annealing temp(°C) |
|---|---|---|---|
A-(F) | GCCTCAGGCCTCCTTATT | 1–18 | 51 |
A-(R) | GAAGGGTAGAAGCATCATAAAC | 14,621,483 | |
B-(F) | AATGGCACTGGCACCTATCC | 1002–1021 | 52 |
B-(R) | CTCCAACCATTCTGCCAAAG | 2338–2357 | |
C-(F) | TTTTGGGCAGTTGGAGGAC | 2123–2141 | 51 |
C-(R) | GTGCTGCTTTACTTGACCTTTGT | 3379–3401 | |
D-(F) | AGGAGCCAAAGTAGCAGGGAC | 3091–3111 | 54 |
D-(R) | TGTTCACAGGCAACACCACC | 4337–4356 | |
E-(F) | CAGCACTAACTCTATGTCTAAAAGG | 4086–4110 | 51 |
E-(R) | TTTCTGAACTAAAAGCAGAGGG | 5193–5214 | |
X-(F) | TTTGCTTGTGGCGCTTGT | 4731–4748 | 49 |
X-(R) | TTTCAGGCACAGCAAGCAAT | 582–601 |