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Table 2 Vaccine and control groups used during vaccination of mice

From: Development of a hexavalent recombinant protein vaccine adjuvanted with Montanide ISA 50 V and determination of its protective efficacy against acute toxoplasmosis

Vaccine and control groups (n: 11)

Dose

Prime dose (day 0)

Boost dose (day 21)

PBS

100 μl PBS / i.p.

100 μl PBS / i.p.

Montanide ISA 50 V

100 μl Montanide (+) 100 μl PBS

100 μl Montanide (+) 100 μl PBS

Hexavalent recombinant protein mixture

Contains 6 different recombinant proteins [rH2 (+) rA4 (+) rE4 (+) rD6 (+) rE6 (+) rH6] in PBS. The amount of each protein is 20 μg in 200 μl PBS.

Contains 6 different recombinant proteins [rH2 (+) rA4 (+) rE4 (+) rD6 (+) rE6 (+) rH6] in PBS. The amount of each protein is 20 μg in 200 μl PBS.

Hexavalent recombinant protein mixture (+) Montanide ISA 50 V

Contains 6 different recombinant proteins [rH2 (+) rA4 (+) rE4 (+) rD6 (+) rE6 (+) rH6] in PBS. The amount of each protein is 20 μg in 100 μl PBS (+) 100 μl Montanide ISA 50 V

Contains 6 different recombinant proteins [rH2 (+) rA4 (+) rE4 (+) rD6 (+) rE6 (+) rH6] in PBS. The amount of each protein is 20 μg in 100 μl PBS (+) 100 μl Montanide ISA 50 V