Table 1 Oligonucleotide primers used for amplification of virulence genes among UPEC isolates
Primer name | Oligonucleotide sequence (5′-3′) | Sizes (bp) | Gene |
|---|---|---|---|
PapC-F | GACGGCACTGCTGCAGGGTGTGGCG | 328 | papC |
Pap-C-R | ATATCCTTTCTGCAGGGATGCAATA | ||
Fim-H-F | TGTACTGCTGATGGGCTGGTC | 564 | fimH |
Fim-H-R | GGGTAGTCCGGCAGAGTAACG | ||
Sfa-F | CTCCGGAGAACTGGGTGCATCTTAC | 410 | Sfa |
Sfa-R | CGGAGGAGTAATTACAAACCTGGCA | ||
Afa-F | GCTGGGCAGCAAACTGATAACTCTC | 750 | Afa |
Afa-R | CATCAAGCTGTTTGTTCGTCCGCCG | ||
HlyA-F | AACAAGGATAAGCACTGTTCTGGCT | 1177 | hlyA |
HlyA-R | ACCATATAAGCGGTCATTCCCGTCA | ||
Cnf1-F | AAGATGGAGTTTCCTATGCAGGAG | 498 | cnf1 |
Cnf1-R | TGGAGTTTCCTATGCAGGAG |