Table 3 Results of various procedures on pleural fluid mononuclear cells
|  | TB cases (n = 32) | Non-TB (n = 9) | ||
|---|---|---|---|---|
HIV+ (n = 26) | HIV- (n = 6) | HIV+ (n = 5) | HIV- (n = 4) | |
AFB microscopy+ n (%) | 3 (12%) | 1 (17%) | 0 (0%) | 0 (0%) |
Culture+ n (%) | 13 (50%) | 0 (0%)* | 0 (0%) | 0 (0%) |
Nested PCR+ n (%) | 15 (58%) | 2 (33%) | 1 (20%) | 0 (0%) |
Real-time PCR+ n (%) | 2 (8%) | 0 (0%) | 0 (0%) | 0 (0%) |
Mycobacterial antigens by immunocytochemistry | ||||
 Heterogeneous antigens+ n (%) | 11 (42%) | 2 (33%) | 4 (80%) | 0 (0%) |
 Cell wall antigen+ n (%) | 10 (38%) | 2 (33%) | 1 (20%) | 1 (25%) |
 LAM+ n (%) | 13 (50%) | 2 (33%) | 0 (0%) | 0 (0%) |
 Secreted antigen+ n (%) | 9 (35%) | 3 (50%) | 2 (40%) | 0 (0%) |
 Any antigen+ n (%) | 19 (73%) | 4 (67%) | 4 (80%) | 1 (25%) |
 LAM or secreted antigen+ n (%) | 14 (54%) | 4 (67%) | 2 (40%) | 0 (0%) |
 LAM and secreted antigen+ n (%) | 8 (31%) | 1 (17%) | 0 (0%) | 0 (0%) |
 Heterogeneous antigens stained cells/100 fields median (range) | 0 (0–348) | 0 (0–66) | 8 (0–31) | 0 (0–0) |
 Cell wall antigen stained cells/100 fields median (range) | 0 (0–130) | 0 (0–19) | 0 (0–1) | 0 (0–1) |
 LAM, stained cells/100 fields median (range) | 0.5 (0–33) | 0 (0–12) | 0 (0–0) | 0 (0–0) |
 Secreted antigen stained cells/100 fields median (range) | 0 (0–72) | 2 (0–12) | 0 (0–5) | 0 (0–0) |
Combinations of antigens and other diagnostic tests | ||||
 Culture and/or n-PCR and/or any antigen+ n (%) | 23 (89%) | 5 (83%) | 5 (100%)* | 1 (25%) |
 Culture and/or n-PCR and/or LAM n (%) | 21 (81%) | 4 (67%) | 1 (20%) | 0 (0%) |