Towards accurate exclusion of neonatal bacterial meningitis: a feasibility study of a novel 16S rDNA PCR assay

Abelian, Arthur; Mund, Thomas; Curran, Martin D.; Savill, Stuart A.; Mitra, Nipa; Charan, Carol; Ogilvy-Stuart, Amanda L.; Pelham, Hugh R. B.; Dear, Paul H.

doi:10.1186/s12879-020-05160-x

BMC Infectious Diseases

Table 1 CSF microscopy, culture and PCRctic results in universal containers (samples 1–52) and snap-top tubes (samples 53–73)

From: Towards accurate exclusion of neonatal bacterial meningitis: a feasibility study of a novel 16S rDNA PCR assay

		Microscopy			Bacteriology
	N^o	WCC^a	RCC^b	R/W^c	Culture	PCRctic
Universal container	1	0	0		no growth	negative
	2	10	662		no growth	negative
	3	26	30,780	1183	no growth	Armatimonadetes
	4	12	6930		no growth	negative
	5	6	32,310		no growth	negative
	6	?	?		no growth	negative
	7	0	9180		no growth	negative
	8	0	260		no growth	Flavobacteriaceae [23] & Rhodobacteraceae
	9	0	6570		no growth	Hymenobacter (Flavobacteriaceae)
	10	0	0		no growth	negative
	11	24	51,120	2130	no growth	Flavobacteriaceae
	12	6	280		no growth	Ochrobactrum sp. [24]
	13	8	54,000		no growth	Mixed sequence
	14	0	137		no growth	Oscillatoriales
	15	0	690		no growth	negative
	16	8	12		no growth	Acinetobacter [25]
	17	24	20	0.8	no growth	negative
	18	22	42	1.9	no growth	negative
	19	2	4680		no growth	negative
	20	14	15,840		no growth	negative
	21	0	24		no growth	negative
	22	6	52		no growth	Sphingomonadaceae [26]
	23	8	14		no growth	Aerococcus christensenii [27] & Sneathia amnii [28]
	24	12	228		no growth	negative
	25	0	2610		no growth	negative
	26	0	140		no growth	negative
	27	10	300		no growth	negative
	28	30	11,430	381	no growth	Sneathia amnii
	29	0	3310		no growth	negative
	30	0	52		no growth	Streptococcaceae [29,30,31]
	31	0	2		no growth	negative
	32	4	146		no growth	negative
	33	16	22,860		no growth	Pseudomonas sp. [12,13,14]
	34	0	9720		no growth	Bdellovibrio
	35	0	10,440		no growth	Staphylococcus [12,13,14]
	36	8	49,500		no growth	negative
	37	8	1000		no growth	Ureaplasma [32]
	38	0	790		no growth	negative
	39	0	1800		no growth	negative
	40	0	40		no growth	negative
	41	0	80		no growth	negative
	42	0	24,800		no growth	negative
	43	4	1126		no growth	negative
	44	0	38		no growth	negative
	45	10	7560		no growth	Cloacibacterium rupense
	46	8	9360		no growth	negative
	47	4	284		no growth	Mixed sequence
	48	0	18,900		no growth	negative
	49	0	0		no growth	negative
	50	0	6		no growth	Methylobacterium sp.
	51	4	10,170		no growth	Pedobacter suwonensis
	52	0	0		no growth	Staphylococcus aureus
Snap-top tube (“Eppendorf”)	53	16	22,860		no growth	negative
	54	0	40		no growth	negative
	55	2	70		no growth	negative
	56	0	3420		no growth	negative
	57	0	0		no growth	negative
	58	2	2088		no growth	negative
	59	42	21,690	516	no growth	negative
	60	0	1080		no growth	negative
	61	0	40		no growth	negative
	62	0	132		no growth	negative
	63	12	22		no growth	Geobacter
	64	4	16		no growth	negative
	65	10	1940		no growth	negative
	66	8	4		no growth	negative
	67	4	36		no growth	Mixed sequence
	68	0	2		no growth	negative
	69	0	8820		no growth	negative
	70	0	8010		no growth	negative
	71	6	4		no growth	negative
	72	51	406,800	7976	no growth	negative
	73	6	12		no growth	negative

^a WCC – CSF White blood cell count, per microlitre
^b RCC – CSF red blood cell count, per microlitre
^c R/W – the ratio of CSF red to white cells (calculated only for samples with ≥20 white cells in microliter [10]. The ratio that allows accurate exclusion of neonatal bacterial meningitis remains uncertain [8]

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ISSN: 1471-2334

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