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Table 1 CSF microscopy, culture and PCRctic results in universal containers (samples 1–52) and snap-top tubes (samples 53–73)

From: Towards accurate exclusion of neonatal bacterial meningitis: a feasibility study of a novel 16S rDNA PCR assay

  Microscopy Bacteriology
NoWCCaRCCbR/WcCulturePCRctic
Universal container100 no growthnegative
210662 no growthnegative
32630,7801183no growthArmatimonadetes
4126930 no growthnegative
5632,310 no growthnegative
6?? no growthnegative
709180 no growthnegative
80260 no growthFlavobacteriaceae [23] & Rhodobacteraceae
906570 no growthHymenobacter (Flavobacteriaceae)
1000 no growthnegative
112451,1202130no growthFlavobacteriaceae
126280 no growthOchrobactrum sp. [24]
13854,000 no growthMixed sequence
140137 no growthOscillatoriales
150690 no growthnegative
16812 no growthAcinetobacter [25]
1724200.8no growthnegative
1822421.9no growthnegative
1924680 no growthnegative
201415,840 no growthnegative
21024 no growthnegative
22652 no growthSphingomonadaceae [26]
23814 no growthAerococcus christensenii [27] & Sneathia amnii [28]
2412228 no growthnegative
2502610 no growthnegative
260140 no growthnegative
2710300 no growthnegative
283011,430381no growthSneathia amnii
2903310 no growthnegative
30052 no growthStreptococcaceae [29,30,31]
3102 no growthnegative
324146 no growthnegative
331622,860 no growthPseudomonas sp. [12,13,14]
3409720 no growthBdellovibrio
35010,440 no growthStaphylococcus [12,13,14]
36849,500 no growthnegative
3781000 no growthUreaplasma [32]
380790 no growthnegative
3901800 no growthnegative
40040 no growthnegative
41080 no growthnegative
42024,800 no growthnegative
4341126 no growthnegative
44038 no growthnegative
45107560 no growthCloacibacterium rupense
4689360 no growthnegative
474284 no growthMixed sequence
48018,900 no growthnegative
4900 no growthnegative
5006 no growthMethylobacterium sp.
51410,170 no growthPedobacter suwonensis
5200 no growthStaphylococcus aureus
Snap-top tube (“Eppendorf”)531622,860 no growthnegative
54040 no growthnegative
55270 no growthnegative
5603420 no growthnegative
5700 no growthnegative
5822088 no growthnegative
594221,690516no growthnegative
6001080 no growthnegative
61040 no growthnegative
620132 no growthnegative
631222 no growthGeobacter
64416 no growthnegative
65101940 no growthnegative
6684 no growthnegative
67436 no growthMixed sequence
6802 no growthnegative
6908820 no growthnegative
7008010 no growthnegative
7164 no growthnegative
7251406,8007976no growthnegative
73612 no growthnegative
  1. a WCC – CSF White blood cell count, per microlitre
  2. b RCC – CSF red blood cell count, per microlitre
  3. c R/W – the ratio of CSF red to white cells (calculated only for samples with ≥20 white cells in microliter [10]. The ratio that allows accurate exclusion of neonatal bacterial meningitis remains uncertain [8]