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Fig. 3 | BMC Infectious Diseases

Fig. 3

From: Towards accurate exclusion of neonatal bacterial meningitis: a feasibility study of a novel 16S rDNA PCR assay

Fig. 3

Sensitivity of PCRctic with 200-μl CSF samples “spiked” with bacteria. Electrophoresis of PCRctic products in 3% agarose gel. aE. coli (DH5-alpha strain) were titrated to an average of 1.5 or 5 CFU/sample. Occasional negative results at 1.5 CFU/sample can be accounted for by the random nature of titration at this level: with an average of 1.5 CFU/sample, approximately 22% of samples should be negative. bStreptococcus pneumoniae, Staphylococcus aureus and Listeria monocytogenes (patient-derived isolates) were titrated by 10-fold dilutions to ≤5 CFU/sample

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