Fig. 5

Effect of simulated hand washing on direct skin-to-skin transfer. a Total amount of pathogen recovered from secondary VITRO-SKIN coupon following direct transfer in the presence or absence of a simulated hand washing event. The CFU transferred (Log10) within mixtures are represented by the sum of the CFU for each pathogen averaged across three replicates. b Total amount of individual ESKAPE+C organisms within the microbial community mixture recovered from the secondary VITRO-SKIN coupon following direct transfer in the presence or absence of a simulated hand washing event. Each pathogen is represented by the mean total CFU for each pathogen across three replicates. Black or gray lines above each point indicate initial pathogen spike levels on the primary VITRO-SKIN coupon. Dotted-line represents the limit of detection. Points below the dotted line result from averaging between replicates when one replicate had zero CFU