Table 1 Sequences of primers sets used and targeted genes

Cryptosporidium parvum/hominis

1PSF

AAC TTT AGC TCC AGT TGA GAA AGT ACT C

hsp70 gene

(Garces-Sanchez, Wilderer et al. 2009)

1PSR

CAT GGC TCT TTA CCG TTA AAG AAT TCC

Blastocystis hominis

BH1

GCT TAT CTG GTT GAT CCT GCC AGT

16 like RNA

(Init, Foead et al. 2007)

BH2

TGA TCC TTC CGC AGG TTC ACC TAC A

Cystoisospora belli

Ib213F

GGA TAT TCC CTG CAG CAT GT

5,8S rRNA/ITS2

(Taniuchi, Verweij et al. 2011)

Ib213R

CGG GAC ACA ACT CAA CAC TG

Encephalitozoon intestinalis

Eint214F

CAC CAG GTT GAT TCT GCC TGA C

SSU rRNA

(Verweij, Ten Hove et al. 2007)

Eint214R

CTA GTT AGG CCA TTA CCC TAA CTA CCA

Enterocytozoon bieneusi

EbITS-89F

TGT GTA GGC GTG AGA GTG TAT CTG

SSU rRNA

(Verweij, Ten Hove et al. 2007)

EbITS-191R

CAT CCA ACC ATC ACG TAC CAA TC

Dientamoeba fragilis

DFpn_1f

GCC AAG GAA GCA CAC TAT GG

SSU rRNA

(Roser, Nejsum et al. 2013)

DFpn_364r

GTA AGT TTC GCG CCT GCT

Trichomonas intestinalis

TH3

TGT AAA CGA TGC CGA CAG AG

SSU rRNA

(Crucitti, Abdellati et al. 2004)

TH5

CAA CAC TGA AGC CAA TGC GAG C

Entamoeba histolytica

EnthF

ATG GCC AAT TCA TTC AAT GA

SSU rRNA

(Suzuki, Kobayashi et al. 2008)

EnthR

TAC TTA CAT AAA GTC TTC AAA ATG T

Entamoeba dispar

EntdF

GTT AGT TAT CTA ATT TCG ATT AGA AC

SSU rRNA

(Suzuki, Kobayashi et al. 2008)

EntdR

ACA CCA CTT ACT ATC CCT ACC TA

Entamoeba hartmanni

EhartF

GTG AAG AGA AAG GAT ATC CAA AGT

SSU rRNA

(Suzuki, Kobayashi et al. 2008)

EhartR

ATA TCA TTT TCA ACT ACG AGC

Ascaris lumbricoides

Alum96F

GTA ATA GCA GTC GGC GGT TTC TT

ITS1

(Wiria, Prasetyani et al. 2010)

Alum183R

GCC CAA CAT GCC ACC TAT TC

Enterobius vermicularis

EVpn_1f

CAA CAC TTG CAC GTC TCT TCA

5S rRNA

(Roser, Nejsum et al. 2013)

EVpn_195r

ATT GCT CGT TTG CCG ATT AT

Trichuris trichiura

TtF

TTG AAA CGA CTT GCT CAT CAA CTT

18S

(Liu, Gratz et al. 2013)

TtR

CTG ATT CTC CGT TAA CCG TTG TC

Strongyloides stercoralis

Stro-1530F

GAA TTC CAA GTA AAC GTA AGT CAT TAG C

18S

(Verweij, Canales et al. 2009)

Stro-1630R

TGC CTC TGG ATA TTG CTC AGT TC

Ancylostoma duodenalis

Ad125F

GAA TGA CAG CAA ACT CGT TGT TG

ITS2

(Verweij, Brienen et al. 2007)

Ad195R

ATA CTA GCC ACT GCC GAA ACG T

Necator americanus

Na58F

CTG TTT GTC GAA CGG TAC TTG C

ITS2

(Verweij, Brienen et al. 2007)

Na158R

ATA ACA GCG TGC ACA TGT TGC

Schistosoma mansoni

Smcyt748F

CCC TGC CAA ATG AAG AGA AAA C

Mitochondrial DNA

(ten Hove, Verweij et al. 2008)

Smcyt847R

TGG GTG TGG AAT TGG TTG AAC

Schistosoma intercalatum

Sh110

TTC CTC CAA CTA CCA TCT TAT CTC

Sh110

(Abbasi, King et al. 2007)

Sm-SL

AAC CGT CAC GGT TTT ACT CTT GTG

Hymenolepsis sp.

Pr-a

TGG TTT TTT GTG CAT CCT GAG GTT TA

COI

(Muehlenbachs, Bhatnagar et al. 2015)

Pr-b

AGA AAG AAC GTA ATG AAA ATG AGC AAC