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Fig. 3 | BMC Infectious Diseases

Fig. 3

From: Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65

Fig. 3

Compare sensitivity of detection of T. vaginalis by nested PCR and LAMP with gradient dilution of T. vaginalis trophozoites. a: Agarose gel electrophoresis of inner amplification products. (Lane M) DL 2000 the DNA molecular weight marker (ordinate values are expressed in bp); (Lane 1–4) The T. vaginalis trophozoites were diluted to 103, 102, 101 and 1, amplified by outer amplification, and then amplified by inner amplification. b: Agarose gel electrophoresis of LAMP products. (Lane M) DL 2000 the DNA molecular weight marker (ordinate values are expressed in bp); (Lane 1–4) The T. vaginalis trophozoites were diluted to 103, 102, 101 and 1. c: LAMP products detected by addition of SYBR GreenI. (Lane 1–4) The T. vaginalis trophozoites were diluted to 103, 102, 101 and 1

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