Fig. 2
Compare sensitivity of detection of T. vaginalis by nested PCR and LAMP with gradient dilution of T. vaginalis DNA template. a: Agarose gel electrophoresis of inner amplification products.(Lane M) DL 2000 the DNA molecular weight marker (ordinate values are expressed in bp); (Lane 1–8) The initial concentration of 90 ng/μL of T. vaginalis DNA was diluted by 101–108 times, amplified by outer amplification, and then amplified by inner amplification. b: Agarose gel electrophoresis of LAMP products. (Lane M) DL 2000 the DNA molecular weight marker (ordinate values are expressed in bp); (Lane 1–7) The initial concentration of 90 ng/μL of T. vaginalis DNA was diluted by 106–1012 times. c: LAMP products detected by addition of SYBR GreenI. (Lane 1–7) The initial concentration of 90 ng/μL of T. vaginalis DNA was diluted by 106–1012 times