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Fig. 1 | BMC Infectious Diseases

Fig. 1

From: Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65

Fig. 1

Detection of T. vaginalis with amplification of actin gene by nested PCR and AP65 gene by LAMP. a: Agarose gel electrophoresis of nested PCR products. (Lane M) DL 2000 the DNA molecular weight marker (ordinate values are expressed in bp); (Lane 1) Negative control outer amplification products; (Lane 2) Negative control inner amplification products; (Lane 3) T. vaginalis DNA outer amplification products; (Lane 4) T. vaginalis DNA inner amplification products. b: Agarose gel electrophoresis of LAMP products. (Lane M) DL 2000 the DNA molecular weight marker (ordinate values are expressed in bp); (Lane 1) Negative control LAMP products; (Lane 2) T. vaginalis DNA LAMP products; c: LAMP products detected by addition of SYBR GreenI. (Lane 1) Negative control LAMP products (orange); (Lane 2) T. vaginalis DNA LAMP products (green)

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BMC Infectious Diseases

ISSN: 1471-2334

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