Prevalence of Zika virus neutralizing antibodies in healthy adults in Vietnam during and after the Zika virus epidemic season: a longitudinal population-based survey

Table 2 Anti-Zika virus immunoglobulin M and G levels of 13 participants with neutralizing antibodies to Zika virus epidemic) (N = 879)

	ELISA results			Neutralizing antibody titer (PRNT₅₀)					Neutralizing antibody titer (PRNT₉₀)
Sample ID	ZIKV IgM(P/N ratio)	DENV IgM(Index Value)	Anti-ZIKV NS1 IgG (P/N ratio)	ZIKV	DENV-1	DENV-2	DENV-3	DENV-4	ZIKV	DENV-1	DENV-2	DENV-3	DENV-4
During epidemic (January 2017)
Z120a^a	3.3	0.1	2.2	160	320	320	< 20	< 20	< 20	320	80	< 20	< 20
Z140a	2.3	0.3	4.7^a	80	40	320	< 20	< 20	< 20	< 20	40	< 20	< 20
Z141a	4.9	0.1	3.4	640	160	80	< 20	< 20	40	80	< 20	< 20	< 20
Z153a^b	2.8	0.3	3.2	160	< 20	< 20	< 20	< 20	40	< 20	< 20	< 20	< 20
Z476a	4.7	0.5	43.9^a	640	320	1280	20	< 20	80	320	160	< 20	< 20
Z735a	2.8	0.6	8.1	320	< 80	< 80	< 80	< 80	80	< 80	< 80	< 80	< 80
Z147a^a	1.6	ND	4.5	320	80	160	20	40	80	< 20	80	< 20	< 20
Z472a	1.5	ND	46.1	5120	160	160	80	40	640	80	40	80	< 20
Z606a	1.6	ND	10.6	1280	< 20	< 20	< 20	< 20	160	< 20	< 20	< 20	< 20
Z138a	4.6	0.6	0.9	40	320	80	< 20	< 20	< 20	160	< 20	< 20	< 20
Z420a^b	3.7	0.1	0.2	160	160	160	20	< 20	20	20	40	< 20	< 20
Z587a	1.2	ND	0.2	320	640	640	1280	160	80	320	320	640	20
Z591a	1.2	ND	0.6	640	640	640	1280	80	80	320	160	640	< 20
Post-epidemic (July 2018)
Z120b^a	ND	ND	2.3	80	640	160	1280	20	20	320	40	320	< 20
Z140b	ND	ND	5.2	640	< 20	160	< 20	20	20	< 20	80	< 20	< 20
Z141b	ND	ND	2.7	640	160	20	160	< 20	80	80	< 20	40	< 20
Z476b	ND	ND	4.7	2560	320	320	320	80	160	80	80	160	< 20
Z735b	ND	ND	3.5	80	320	80	320	80	< 20	320	20	160	20
Z138b	ND	ND	0.5	80	40	< 20	40	< 20	< 20	20	< 20	20	< 20

DENV dengue virus, DENV1–4 dengue virus serotypes 1–4, ELISA enzyme-linked immunosorbent assay, IgG immunoglobulin G, IgM immunoglobulin M, N negative, ND not detected, P positive, PRNT₅₀ plaque-reduction neutralization test with neutralization defined as ≥50% reduction in challenge virus plaque-forming units, PRNT₉₀ plaque-reduction neutralization test with neutralization defined as ≥90% reduction in challenge virus plaque-forming units, ZIKV Zika virus
Figures in bold indicate positive results_.^a ZIKV antibodies were determined using anti-ZIKV NS1 IgG ELISA (P/N ratio ≥ 2). ^b Z153 and Z420 were not available during the post endemic collection

ISSN: 1471-2334