Fig. 2

Flow cytometric analysis demonstrated the efficacies of Th17 neutrilization in vivo. The proportion of Th17 in CD4⁺ T cells (a), absolute cell numers of Th17 (b), and dot plots (c) after anti-IL-17 mAb treatment were detected. Column diagram (left) and representative dot plots (right) of Th17 in the infected with P.y17XL (normal infection) and CD25-depleted BALB/c P.y17XL (P.y17XL + anti-IL-17 mAb) were displayed. Results are presented as arithmetic mean of 4 mice per group ± SE. Single asterisk (*) and double asterisks (**) indicates P < 0.05 and P < 0.01, respectively, compared with control mice (non-infected mice, 0d). Single pound sign (^#) and double pound sign (^##) indicates P < 0.05, and P < 0.01, respectively, between infected and Tregs depletion groups mice.* = Calculated value of ‘p’ by student’s t-test; # = Calculated value of ‘p’ by one-way ANON. P-values were calibrated using Bonferroni correction. The data are representative of three separate experiments