Fig. 1

Flow cytometric analysis demonstrated the efficacies of Tregs depletion in vivo. The proportion of Tregs in CD4⁺ T cells (a), absolute cell numbers of Tregs (b), and dot plots (c) before and after anti-7D4 mAb treatment were detected. Column diagram (left) and representative dot plots (right) of Tregs in the infected with P.y17XLP.y17XL (normal infection) and CD25-depleted BALB/c P.y17XL (P.y17XLP.y17XL + 7D4) were displayed. Results are presented as arithmetic mean of 4 mice per group ± SE. Single asterisk (*) and double asterisks (**) indicates P < 0.05 and P < 0.01, respectively, compared with control mice (non-infected mice, 0d). Single pound sign (^#) and double pound sign (^##) indicates P < 0.05, and P < 0.01, respectively, between infected and Tregs depletion groups mice. * = Calculated value of ‘p’ by student’s t-test; # = Calculated value of ‘p’ by one-way ANON. P-values were calibrated using Bonferroni correction.The data are representative of three separate experiments