Skip to main content

Table 1 The primers used in this study

From: Molecular identification and antifungal susceptibility profile of yeast from vulvovaginal candidiasis

Candida species

Primer name

Forward(5′-3′)

Reverse(5′-3′)

Amplified fragment size (bp)

References

C. albicans complexes

HWP1

GCTACCACTTCAGAATCATCATC

GCACCTTCAGTCGTAGAGACG

C. albicans:839 and 941

C. africana:700

C. dubliniensis:569

Shan,2014

C. glabrata complexes

GLA

NIV

BRA

CGGTTGGTGGGTGTTCTGC

AGGGAGGAGTTTGTATCTTTCAAC

GGGACGGTAAGTCTCCCG

ACCAGAGGGCGCAATGTG

C. glabrata:397

C. bracarensis: 223

C. nivariensis:293

Li,2014

C. parapsilosis complexes

mCPF

mCOF

mCMF

TTTGCTTTGGTAGGCCTTCTA

TAAGTCAACTGATTAACTAAT

AACTGCAATCCTTTTCTTTCTA

AATATCTGCAATTCATATTACT

C. parapsilosis:171

C. orthopsilosis:109

C. metapsilosis:217

Asadzadeh,2015

Rare yeast

NL1,NL4

GCATATCAATAAGCGGAGGAAAAG-3’

GGTCCGTGTTTCAAGACGG

500–600

Leaw,2006

  1. 1Shan Y, Fan S, Liu X, et al. Prevalence of Candida albicans-closely related yeasts, Candida africana and Candida dubliniensis, in vulvovaginal candidiasis. Med Mycol, 2014, 52 (6): 636–40.
  2. 2Li J, Shan Y, Fan S, et al. Prevalence of Candida nivariensis and Candida bracarensis in vulvovaginal Candidiasis. Mycopathologia, 2014, 178 (3, 4): 279–83.
  3. 3Asadzadeh M, Ahmad S, Hagen F, et al. Simple, Low-Cost Detection of Candida parapsilosis complex isolates and molecular fingerprinting of Candida orthopsilosis strains in Kuwait by ITS region sequencing and amplified fragment length polymorphism analysis. PLoS One, 2015, 10 (11): e0142880.
  4. 4Leaw SN, Chang HC, Sun HF, et al. Identification of medically important yeast species by sequence analysis of the internal transcribed spacer regions. J Clin Microb, 2006, 44 (3): 693–9.