Table 1 Comparison of in house and commercial Real Time PCR kits targeting T. gondii RE gene in 38 clinical samples
Sample type | in house Real Time PCR | Commercial Real Time PCR | Compatibility | Number of sample with the same PCR results |
|---|---|---|---|---|
Peripheral blood (Buffy coat) | −/− +/+ −/− +/− | −/− +/+ +/+ +/+ | Compatible Compatible Partly compatible Completely incompatible | 8 2 1 1 |
Amniotic fluid | −/− +/+ | −/− +/+ | Compatible Compatible | 10 1 |
Tissue | −/− +/+ | −/− +/+ | Compatible Compatible | 6 3 |
Cerebrospinal fluid (CSF) | −/− | −/− | Compatible | 5 |
Intraocular fluid | −/− | −/− | Compatible | 1 |
TOTAL | 38 |