Table 1 – Specific enteric pathogen primers and probes for TaqMan Array Card used to determine the primary outcome

Aeromonas

Aerolysin

TYCGYTACCAGTGGGACAAG

CCRGCAAACTGGCTCTCG

CAGTTCCAGTCCCACCACTT

[2]

Campylobacter jejuni/C. coli

cadF

CTGCTAAACCATAGAAATAAAATTTCTCAC

CTTTGAAGGTAATTTAGATATGGATAATCG

CATTTTGACGATTTTTGGCTTGA

[2]

Clostridium difficile

tcdB

GGTATTACCTAATGCTCCAAATAG

TTTGTGCCATCATTTTCTAAGC

CCTGGTGTCCATCCTGTTTC

[2]

Enteroaggregative Escherichia coli (EAEC)

aaiC

ATTGTCCTCAGGCATTTCAC

ACGACACCCCTGATAAACAA

TAGTGCATACTCATCATTTAAG

[2]

Enteroaggregative Escherichia coli (EAEC)

aatA

CTGGCGAAAGACTGTATCAT

TTTTGCTTCATAAGCCGATAGA

TGGTTCTCATCTATTACAGACAGC

[2]

Enterohemorrhagic E. coli (EHEC) 0157

rdbE

TTTCACACTTATTGGATGGTCTCAA

CGATGAGTTTATCTGCAAGGTGAT

CTCTCTTTCCTCTGCGGTCCT

[1]

Enteropathogenic

E. coli (EPEC)

eae

CATTGATCAGGATTTTTCTGGTGATA

CTCATGCGGAAATAGCCGTTA

ATACTGGCGAGACTATTTCAA

[2]

Enteropathogenic

E. coli (EPEC)

bfpA

TGGTGCTTGCGCTTGCT

CGTTGCGCTCATTACTTCTG

CAGTCTGCGTCTGATTCCAA

[2]

Enterotoxigenic

E. coli (ETEC) LT toxin

ETEC LT

TTCCCACCGGATCACCAA

CAACCTTGTGGTGCATGATGA

CTTGGAGAGAAGAACCCT

[2]

Enterotoxigenic

E. coli (ETEC) ST toxin

STh STp

GCTAAACCAGYAGRGTCTTCAAAATGAATCACTTGACTCTTCAAAA

CCCGGTACARGCAGGATTACAACATGAATCACTTGACTCTTCAAAA

TGGTCCTGAAAGCATGAATGAACAACACATTTTACTGCT

[2]

Salmonella enteritidis

ttr

CTCACCAGGAGATTACAACATGG

AGCTCAGACCAAAAGTGACCATC

CACCGACGGCGAGACCGACTTT

[2]

Shigella spp.

virG

TCAGAAAGGTAATTGGCATGGA

AGAACCGCGCCCAAAGA

AGGGCGGAATATT

[1]

Vibrio cholerae

hlyA

ATCGTCAGTTTGGAGCCAGT

TCGATGCGTTAAACACGAAG

ACCGATGCGATTGCCCAA

[2]

MS2

MS2g1

TGGCACTACCCCTCTCCGTATTCAC

GTACGGGCGACCCCACGATGAC

CACATCGATAGATCAAGGTGCCTACAAGC

[2]

Adenovirus 40–41

Fiber Gene

AACTTTCTCTCTTAATAGACGCC

AGGGGGCTAGAAAACAAAA

CTGACACGGGCACTCT

[2]

Adenovirus broad species

Hexon

GCCACGGTGGGGTTTCTAAACTT

GCCCCAGTGGTCTTACATGCACATC

TGCACCAGACCCGGGCTCAG

[1]

Norovirus GI

ORF 1–2

CGYTGGATGCGNTTYCATGA

CTTAGACGCCATCATCATTYAC

TGGACAGGAGATCGC

[1]

Norovirus GII

ORF 1–2

CARGARBCNATGTTYAGR TGGATGAG

TCGACGCCATCTTCATTCACA

TGGGAGGGCGATCGCAATCT

[2]

Rotavirus

NSP3

ACCATCTWCACRTRACCCTCTATGAG

GGTCACATAACGCCCCTATAGC

AGTTAAAAGCTAACACTGTCAAA

[2]

Giardia duodenalis Assemblage A

triosephosphate isomerase (TPI)

TTCCGCCGTACACCTGTC

GCGCTGCTATCCTCAACTG

ATTGCGGCAAACACGTCA

[1]

Giardia duodenalis Assemblage B

triosephosphate isomerase (TPI)

GATGAACGCAAGGCCAATAA

CTTTGATTCTCCAATCTCCTTCTT

AATATTGCTCAGCTCGAGGC

[1]

Cryptosporidium spp.

18 s rRNA

GGGTTGTATTTATTAGATAAAGAACCA

AGGCCAATACCCTACCGTCT

TGACATATCATTCAAGTTTCTGAC

[2]

C. hominus

LIB13

TCCTTGAAATGAATATTTGTGACTCG

AAATGTGGTAGTTGCGGTTGAAA

CTTACTTCGTGGCGGCGT

[1]

C. parvum

LIB13

TCCTTGAAATGAATATTTGTGACTCG

TTAATGTGGTAGTTGCGGTTGAAC

TATCTCTTCGTAGCGGCGTA

[1]