Table 1 Primers used for Quantitative Real-Time PCR
From: Inhibition of type I interferon signaling abrogates early Mycobacterium bovis infection
Gene name | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
Ifnb1 | AAGAGTTACACTGCCTTTGCCAT | CACTGTCTGCTGGTGGAGTTCATC |
Ifnar1 | CTCCATCTCCTGCTTAGTGT | ATTAGTTACTGTGGGTACTTGTG |
Irf7 | ACTTCAGCACTTTCTTCCGAGAAC | AGGTAGATGGTGTAGTGTGGTGAC |
Oas2 | AAAGTCCTGAAGACCGTCAAGGG | ACAACAATGTCAGCATCTGATCCC |
Igs15 | AGCAATGGCCTGGGACCTAAAG | AGTCACGGACACCAGGAAATCG |
Ifng | AGCAACAACATAAGCGTCAT | CCTCAAACTTGGCAATACTC |
Tnf | CAAAATTCGAGTGACAAGCCTGT | CCACTTGGTGGTTTGCTACGA |
Nos2 | TCCTCACGCTTGGGTCTTGTTC | TCCAACGTTCTCCGTTCTCTTGC |
Mrc1 | AATGCCAAAAATTATTGATCGTG | ACGGTGACCACTCCTGCTG |
Ym1 | TACCCTATGCCTATCAGGGTAAT | CCTTGAGCCACTGAGCCTTC |
Arg1 | AATGAAGAGCTGGCCTGGTGTGGT | ATGCTTCCAACTGCCAGACTGTG |
T-bet | CCCCTGTCCAGTCAGTAACTT | CTTCTCTGTTTGGCTGGCT |
Gata-3 | AAGAAAGGCATGAAGGACGC | GTGTGCCCATTTGGACATCA |
Foxp3 | CTTCACCAAGGTGAGCGAGT | CTTCTGTCTGGAGTGGCTGG |
β-actin | CCTTCTGACCCATTCCCACC | GCTTCTTTGCAGCTCCTTCG |