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Fig. 5 | BMC Infectious Diseases

Fig. 5

From: Inhibition of type I interferon signaling abrogates early Mycobacterium bovis infection

Fig. 5

Type I IFNs drive macrophage polarization toward an anti-inflammatory profile in M. bovis-infected lungs. a-b BMDMs were infected with M. bovis at a multiplicity of infection (MOI) of 10 in the presence or absence of the αIFNAR1 antibody. Relative expression of macrophage M2 (Arg1, Ym1, and Mrc1) and M1 (Nos2 and Tnf) polarization gene markers were quantified by qRT-PCR at 24 hpi. Data were normalized to housekeeping gene β-actin. Mice were infected with M. bovis as mentioned above (Fig. 4). c-e) qRT-PCR was performed for quantification of M2 (Arg1, Ym1, and Mrc1) and (f-h) M1 (Ifng, Nos2 and Tnf) polarization markers in the lungs of infected mice. The effect of αIFNAR1 antibody treatment on polarization of macrophages in the lung tissues of mice infected with M. bovis at 21 days p.i. Representative flow cytometric plots of CD86-expressing (M1) (i-j) and CD206-expressing (M2) macrophages and relative cell numbers (k-l). Each bar represents mean ± SD (n = 5). Data are a pool of two independent experiments

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