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Fig. 4 | BMC Infectious Diseases

Fig. 4

From: Inhibition of type I interferon signaling abrogates early Mycobacterium bovis infection

Fig. 4

Type I IFNs regulates lung myeloid cell recruitment during M. bovis infection. Mice treated with αIFNAR1 antibody or isotype antibody followed by infection with M. bovis. Myeloid cell populations in infected lung tissues were analyzed by flow cytometry at 21 days p.i. a Lung cells were gated on single live cells using forward and side scatter parameters. Gating strategy for quantification of myeloid cell populations is shown. R1 (CD11blo CD11c+): alveolar macrophages; R2 (CD11bhi CD11c+): myeloid dendritic cells (DCs); gating on R3 (CD11bhi CD11cneg), R4 (CD11bhi CD11cneg Ly6Clo/neg): recruited macrophages; R5 (CD11bhi CD11cneg Ly6Cint/hi Ly6Gneg): monocytes; and R6 (CD11bhi CD11cneg Ly6Cint Ly6Ghi): neutrophils. b-f Quantification of myeloid cells phenotypes. Each bar represents mean ± SD (n = 5). Data are a pool of two independent experiments. g H&E staining of lung sections were performed to measure neutrophils infiltration (scale bar: 10 μm). Arrows: neutrophils. h The number of neutrophils infiltrating the lungs of αIFNAR1 mice was evaluated at 21 days p.i. (I) Expression of MHC class II on myeloid DCs in the lungs of mice at 21 days p.i. Data are expressed as MFI ± SE (n = 3). j-l Expression of costimulatory molecules, (j) CD40, (k) CD80, and (l) CD86 on myeloid DCs in the lungs of mice at 21 days p.i; Data are expressed as MFI ± SE (n = 3). (* P < 0.05; ** P < 0.01)

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