Fig. 2

IFNAR1 blockade reduces the severity of M. bovis infection in mice. C57BL/6 mice were treated with αIFNAR1 antibody or isotype antibody followed by infection with 100 CFUs of M. bovis via i.n route. a Body weights of mice with IFNAR1 antibody or mouse IgG1 isotype antibody (n  =  6 mice). Data presented from two experiments. b Survival curve (obtained from 2 independent trials, n  =  18 for mice with mouse IgG1 isotype control antibody, n  =  18 for mice with IFNAR1 antibody); P  < 0.001 compared to mice with IgG1 isotype antibody, analyzed by Log-rank (Mantel-Cox) test. c and d Bacterial load from lung and spleen at 21 and 84 days pi; data shown are representative of two independent trials, n  =  6 mice per group. e Acid-fast staining of lung sections of M. bovis infected mice (scale bar: 10 μm). f and g H&E staining of paraffin-embedded tissue collected at 21 and 84 days p.i for histopathology analysis (scale bar, 2 mm; n = 5). Data is presented as mean ± SD from two independent experiments