Fig. 1
From: Inhibition of type I interferon signaling abrogates early Mycobacterium bovis infection
M. bovis induces type I interferon in C57BL/6 mice. (b-e)Wild type female C57BL/6 mice were challenged with M. bovis (100 CFU) or PBS by intranasal (i.n) route. Mice (n = 3) from each group were analyzed for gene and protein expression at 21 and 84 days post-infection. (a) Ifnb1 and (b) Ifnar1 mRNA expression in the lung tissues were checked by qRT-PCR. Gene expression values were normalized to the housekeeping gene β-actin. (c) IFN-β and (d) IFNAR1 expression in the lungs were detected by IHC method; scale bars, 20 μm. Original magnification, 100×. (e) The level of IFN-β in the blood serum was determined by ELISA. mRNA isolated from lung tissues was probed for the expression of indicated genes by qRT-PCR at (f) 21 dpi and (g) 84 dpi. (h) J774A.1 and (i) BMDM cells were infected with M. bovis at a multiplicity of infection (MOI) of 10 for the indicated time, mRNA was isolated and probed for the expression of Ifnb1 gene by qRT-PCR. Data were normalized to housekeeping gene β-actin. Data was obtained from three independent experiments for in vitro study. Data is presented as mean±SD (* P < 0.05; ** P < 0.01; *** P < 0.001)