Mitigation strategy | Lowa to Mediumb-risk laboratory activities | High-risk laboratory activities c |
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Personnel protection | Gown Gloves Eye protection Covered shoes Avoidance of sharps | Respiratory protection Gown Gloves Eye protection Covered shoes Avoidance of sharps |
Primary containment | Class II BSC (annually certified) Aerosol generating procedures (i.e., centrifugation) devices with bioseal (gaskets buckets and corresponding lids (screw or clip type). Buckets may only be opened in the BSC | Class II BSC (annually certified) Aerosol generating procedures (i.e., centrifugation) devices with bioseal (gaskets buckets and corresponding lids (screw or clip type). Buckets may only be opened in the BSC |
Secondary biocontainment | Biosafety Level 2 or Core | Biosafety Level 3 or Heightened Control Measures |
Disinfection (any of the following options) | Autoclave – 121 °C for 30 min Dry heat - 160-170 °C for at least 60 min Chemical (at least 30 min contact time) 1% Virkon (surfaces) or 2% Virkon (liquids) 1% sodium hypochlorite 70% ethanol Glutaraldehyde Formaldehyde Quaternary ammonium disinfectants | Autoclave – 121 °C for 30 min Dry heat - 160-170 °C for at least 60 min Chemical (at least 30 min contact time) 1% Virkon (surfaces) or 2% Virkon (liquids) 1% sodium hypochlorite 70% ethanol Glutaraldehyde Formaldehyde Quaternary ammonium disinfectants |
Training | Staff need to be trained and demonstrate proficiency in GMPP | Staff need to be trained and demonstrate proficiency in GMPP |