Fig. 4

Cytokines produced by CD103 expressing CD4⁺ and CD8⁺ T cells. Mice were percutaneous infected by S. japonicum. 5–6 week later, lungs were picked out. Single pulmonary cell suspensions were separated from naive and infected mice, and stimulated with PMA plus ionomycin, the expression of IL-4, IFN-γ, IL-9 and IL-10 were detected by intracellular cytokine staining as described in materials and methods. The average expression of IL-4, IFN-γ, IL-9 and IL-10 on CD4⁺ and CD8⁺ CD103⁺ T cells were calculated from FACS data, respectively. Data was from three independent experiments with 5–6 mice per group and shown as mean ± SEM. Variable of the data was analyzed by the Tukey’s HSD. * for P < 0.05